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PRI-8800 Automatic Varying Temperature Incubations and Continuous Soil Respiration Measurements System
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       A reliable and precise estimate of the temperature sensitivity (Q10) of soil organic matter (SOM) decomposition is critical to predict feedbacks between the global carbon cycle and climate change.
       Traditional methods for estimating Q10 includes CDM mode (constant temperature incubation and discontinuous measurements) and VDM mode (varying temperature incubation and discontinuous measurements). According the conclusion of Robinson(2017),no less than 20 temperature points could get more precision Q10. Which is difficult for traditional measurement. Combining rapidly VCM mode (varying temperature incubations and continuous measurements), PRI-8800 (patented) leads a new method for Q10 estimation. VCM mode eliminates the underestimated errors by both CDM and VDM modes, provide a more accurate and rapid estimation of the temperature response of SOM decomposition and can be used for large-scale estimation of Q10.
       PRI-8800 is a new and exciting solution for continuous soil respiration measurements combining varying temperature incubations in lab. Excellent compatibility and extensibility with other analyzers such as various GHGs and trace gas concentration analyzers, various isotope analyzers and other gas measurements devices. Applications are related to soil respiration, biodegradability of plastics in solid medium, biodegradability of plastics in aqueous medium, organic waste (solid or liquid samples), food industry, compost biological activity, wastewaters, R&D in biotechnology, biology, ecology and pharmacy.

Key Feature
  • Varying temperature incubations and continuous measurements;
  • Excellent compatibility and extensibility with various analyzers;
  • Automatic temperature control (-20 ~ 60℃);
  • Temperature fluctuation is better than 0.05℃;
  • Dual gas circuit to eliminate effect of initial high concentration;
  • Inherent cahnnels for isotope and concentration calibration.

Specifications
Parameter Specifications
Flask Capacity 150 mL
Tray Capacity 25 samples
Temperature Range -20 ~ 60℃
Temperature Fluctuation ± 0.05℃
ACC Temperature + 40℃
Refrigerating Capacity @ 20℃ BT/20°C AT 2000W
Heating Rate / Cooling Rate (5-30℃) 1℃/min
Dimensions of Water Bath (Inside) 400 mm W × 400 mm D × 200 mm H
Autosampler Precision 0.02 mm
Air Temperature Precision ±0.15℃ (Temp. sensor)
Pressure Precision ±0.05% (sensor)
Flow Rate 1 L/min
Gas Tube 1/8” Stainless or Teflon
CO2 Absorption Soda lime
System Response time < 4 s
Calibration Channels (option) 3
Interface Android Pad
Power 100 ~ 240VAC,50/60 Hz,
1500 W(Heating); 1250 W (Cooling)
Dimensions 762 mm × 950 cm × 1700 mm
 
8800-1 CO2 H2O analzyer (Integrated in PRI-8800)
CO2 Accuracy ±2%
CO2 Measurement Range 0-2000 ppm
H2O Precision (Typical) ±2%
H2O Measurement Range 0~100% RH
Sampling Temperature -10 ~ 45 °C
Sampling Pressure 80 ~ 115 kPa
Sampling Humidity 0-100% R.H, non-condensing
Fittings 1/4″ Swagelok

Configuration
PRI-8800 includes water bath with refrigerator and heater system, autosampler, 25 sample tray, 50 flasks, control box.
8800-1: stanard CO2 H2O analyzer with 2% CO2 accuracy.
Ultra CO2 N2O H2O analyzer from Aeris Technologies.

Publications
1.Li, C., Xiao, C.W., Guenet, B., Li, M.X., Xu, L., He, N.P. 2022. Short-term effects of labile organic C addition on soil microbial response to temperature in a temperate steppe. Soil Biology and Biochemistry 167, 108589. https://doi.org/10.1016/j.soilbio.2022.108589.
2.Jiang ZX, Bian HF, Xu L, He NP. 2021. Pulse effect of precipitation: spatial patterns and mechanisms of soil carbon emissions. Frontiers in Ecology and Evolution, 9: 673310.
3.Liu Y, Xu L, Zheng S, Chen Z, Cao YQ, Wen XF, He NP. 2021. Temperature sensitivity of soil microbial respiration in soils with lower substrate availability is enhanced more by labile carbon input. Soil Biology and Biochemistry, 154: 108148.
4.Bian HF, Zheng S, Liu Y, Xu L, Chen Z, He NP. 2020. Changes in soil organic matter decomposition rate and its temperature sensitivity along water table gradients in cold-temperate forest swamps. Catena, 194: 104684.
5.Xu M, Wu SS, Jiang ZX, Xu L, Li MX, Bian HF, He NP. 2020. Effect of pulse precipitation on soil CO2 release in different grassland types on the Tibetan Plateau. European Journal of Soil Biology, 101: 103250.
6.Liu Y, He NP, Xu L, Tian J, Gao Y, Zheng S, Wang Q, Wen XF, Xu XL, Yakov K. 2019. A new incubation and measurement approach to estimate the temperature response of soil organic matter decomposition. Soil Biology & Biochemistry, 138, 107596.
7.Liu Y, He NP, Wen XF, Xu L, Sun XM, Yu GR, Liang LY, Schipper LA. 2018. The optimum temperature of soil microbial respiration: Patterns and controls. Soil Biology and Biochemistry, 121: 35-42.
8.Liu Y, Wen XF, Zhang YH, Tian J, Gao Y, Ostle NJ, Niu SL, Chen SP, Sun XM, He NP. Widespread asymmetric response of soil heterotrophic respiration to warming and cooling. Science of Total Environment, 635: 423-431.
9.Wang Q, He NP, Xu L, Zhou XH. 2018. Important interaction of chemicals, microbial biomass and dissolved substrates in the diel hysteresis loop of soil heterotrophic respiration. Plant and Soil, 428: 279-290.
10.Wang Q, He NP, Xu L, Zhou XH. 2018. Microbial properties regulate spatial variation in the differences in heterotrophic respiration and its temperature sensitivity between primary and secondary forests from tropical to cold-temperate zones. Agriculture and Forest Meteorology, 262, 81-88.
11.Li J, He NP, Xu L, Chai H, Liu Y, Wang DL, Wang L, Wei XH, Xue JY, Wen XF, Sun XM. 2017. Asymmetric responses of soil heterotrophic respiration to rising and decreasing temperatures. Soil Biology & Biochemistry, 106: 18-27.
12.Liu Y, He NP, Xu L, Niu SL, Yu GR, Sun XM, Wen XF. 2017. Regional variation in the temperature sensitivity of soil organic matter decomposition in China’s forests and grasslands. Global Change Biology, 23: 3393-3402.
13.Wang Q, He NP*, Liu Y, Li ML, Xu L. 2016. Strong pulse effects of precipitation event on soil microbial respiration in temperate forests. Geoderma, 275: 67-73.
14.Wang Q, He NP, Yu GR, Gao Y, Wen XF, Wang RF, Koerner SE, Yu Q*. 2016. Soil microbial respiration rate and temperature sensitivity along a north-south forest transect in eastern China: Patterns and influencing factors. Journal of Geophysical Research: Biogeosciences, 121: 399-410.
15.He NP, Wang RM, Dai JZ, Gao Y, Wen XF, Yu GR. 2013. Changes in the temperature sensitivity of SOM decomposition with grassland succession: Implications for soil C sequestration. Ecology and Evolution, 3: 5045-5054.
16.He N P, Liu Y, Xu L, Wen X F, Yu G R, Sun X M. Temperature sensitivity of soil organic matter decomposition:New insights into models of incubation and measurement. Acta Ecologica Sinica, 2018, 38(11): 4045-4051.
17.Mao X1, Zheng J1, Yu W, Guo X, Xu K, Zhao R, Xiao L, Wang M, Jiang Y, Zhang S, Luo L, Chang J, Shi Z, Luo Z* 2022. Climate-induced shifts in composition and protection regulate temperature sensitivity of carbon decomposition through soil profile. Soil Biology and Biochemistry 172, 108743.
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