PRI-8800 Plus 全自动变温培养土壤温室气体在线测量系统主要用于在线测量原状土变温条件下的温室气体排放，具有较大单体样本容量，具有可选的土壤温度、湿度、盐度、水势、水位传感器，可以进行包含原状和非原状土壤冻融过程模拟、湿地淹水深度模拟、温度敏感性（Q₁₀）测量、水分敏感性测量、底物敏感性测量、生物敏感性测量等；此外，选配温度、湿度和盐度传感器后，PRI-8800 Plus 可以用于测量水泥、混凝土等在不同温度和不同相态下的含水量和电导率（电阻率）。

       PRI-8800 Plus 具有灵活的兼容性，可以选配不同的气体分析仪，如经济型CO₂ H₂O分析仪、高精度CO₂ CH₄ N₂O H₂O分析仪、CO₂ CH₄同位素分析仪等。

       PRI-8800 Plus全自动变温培养土壤温室气体在线测量系统主要包含自动进样器、水槽、压缩机、CO₂ H₂O 分析仪、内部计算机、9位样品盘等，9个原状测量套件；可选土壤温度传感器、土壤湿度和盐度传感器、土壤水势传感器、水位传感器等。

       PRI-8800 Plus可以选配不同的气体分析仪，如CO₂ H₂O分析仪、高精度CO₂ CH₄ N₂O H₂O分析仪、CO₂ CH₄同位素分析仪等，具体请咨询销售人员。

1）原状土冻融过程模拟：气候变化改变了土壤干湿循环和冻融循环的频率和强度。这些波动影响了土壤微生物活动的关键驱动力，即土壤水分利用率。虽然这些波动使土壤微生物结构有少许改变，但一种气候波动的影响（例如干湿交替）是否影响了对另一种气候（例如冻融交替）的反应，其温室气体排放是如何响应的？通过PRI-8800 Plus 的冻融模拟，我们可以找出清晰答案。

2）湿地淹水深度模拟：在全球尺度上湿地甲烷（CH₄）排放的温度敏感性大小主要取决于水位变化，而二氧化碳（CO₂）排放的温度敏感性不受水位影响。复杂多样的湿地生态系统不同水位的变化及不同温度的变化如何影响和调控着湿地温室气体的排放？我们该如何量化不同水位的变化及不同温度的变化下湿地的温室气体排放？借助PRI-8800 Plus，通过淹水深度和温度变化的组合测试，可以查出真相。

3）温度依赖性的研究：既然温度的变化会极大影响土壤呼吸，基于温度变化的Q₁₀研究成为科学家研究中重中之重。2017年Robinson提出的最低20个温度梯度拟合土壤呼吸对温度响应曲线的建议，将纠正以往研究人员只设置3-5个温度点(大约相隔5-10℃)进行呼吸测量的做法，该建议能解决传统方法因温度梯度少而导致的不同土壤的呼吸对温度变化拟合相似度高的问题，更能提升不同的理论模型或随后模型推算结果的准确性。而上述至少20个温度点的设置和对应的土壤呼吸测量，仅仅需要在PRI-8800 Plus程序中预设几个温度梯度即可完成多个样品在不同温度下的自动测量，这将极大提高科学家的工作效率。

除了上述变温应用案例外，科学家还可以依据自己的实验设计进行诸如日变化、月变化、季节变化、甚至年度温度变化的模拟培养，通过PRI-8800 Plus的“傻瓜式”操作测量，将极大减少科学家实验实施的周期和工作量，并提高了工作效率。

PRI-8800 Plus除了具有上述变温培养的特色，还可以进行恒温培养，抑或是恒温/变温交替培养，这些组合无疑拓展了系统在不同温度组合条件下的应用场景。

4）水分依赖性的研究：多数研究表明，在温度恒定的情况下，Q₁₀很容易受土壤含水量的影响，表现出一定的水分依赖特性。PRI-8800 Plus可以通过手动调整土壤含水量的做法，并在PRI-8800 Plus快速连续测量模式下，实现不同水分梯度条件下土壤呼吸的精准测量，而PRI-8800 Plus的逻辑设计，为短期、中期和长期湿度控制条件下的土壤呼吸的连续、高品质测量提供了可能。

5）底物依赖性的研究：底物物质量与Q₁₀密切相关，这里的底物包含不限于自然态的土壤，如含碳量，含氮量，易分解/难分解的碳比例、土壤粘粒含量、酸碱盐度等；也可能包含了某些外源底物，如外源的生物质碳、微生物种群、各种肥料、呼吸促进/抑制剂、同位素试剂等。通过PRI-8800快速在线变温培养测量，能加速某些研究进程并获得可靠结果，如生物质炭在土壤改良过程中的土壤呼吸研究、缓释肥缓释不同阶段对土壤呼吸的持续影响、盐碱土壤不同改良措施下的土壤呼吸的变化响应等等。

6）生物依赖性的研究：土壤呼吸包含土壤微生物呼吸（>90%）和土壤动物呼吸（1-10%），土壤微生物群落对Q₁₀影响重大。通过温度响应了解培养前后的微生物种群和数量的变化以及对应的土壤呼吸速率的变化有重要意义。外源微生物种群的添加，或许帮助科学家找出更好的Q₁₀对土壤生物依赖性的响应解析。

2026年

1.Yan J, Zheng J, Zhang S, et al. Dissolved organic carbon-mediated controls dominate soil carbon mineralization in response to freeze-thaw cycles[J]. EGUsphere, 2026, 2026: 1-25.

2.Zhao S, Chai H, Li M, et al. Low substrate concentration intensifies the earthworm-driven increase in temperature sensitivity of SOM decomposition[J]. Biology and Fertility of Soils, 2026: 1-14.

3.Wan Y, Xie R, Liu X, et al. Effectiveness and mechanism of microbial remediation of heavy metal-contaminated soil by Bacillus thuringiensis and Acinetobacter calcoaceticus[J]. Journal of Hazardous Materials Advances, 2026: 101116.

4.Huang C, Sáez‐Sandino T, Zhou G, et al. Warming‐Induced Carbon Vulnerability in Permafrost Forests: A Shift in Q 10 From Continuous to Discontinuous Zones[J]. Global Change Biology, 2026, 32(3): e70787.

2025年

5.Zhou Z, Zhang N, Wang Y, et al. Litter regulates the priming effect of carbon mineralization and its temperature sensitivity during freeze–thaw cycles in alpine swamp meadow soils[J]. Plant and Soil, 2025: 1-19.

6.Wang H, Jing H, Ma H, et al. Interactions between fine root-derived dissolved organic matter and K-strategy-dominated soil microbes regulate soil CO2 emissions in a Pinus tabulaeformis plantation under N deposition[J]. Soil and Tillage Research, 2026, 256: 106878.

7.Gao M , Hu W , Li M ,et al. Response of soil basal respiration rates, microbial attributes, and organic matter composition to land‐use change[J].Soil Science Society of America Journal, 2025, 89(2).DOI:10.1002/saj2.70052.

8.Zheng J , Groenigen K J V , Hartley I P ,et al. Temperature sensitivity of bacterial species-level preferences of soil carbon pools[J]. Geoderma, 2025, 456. DOI:10.1016/j.geoderma.2025.117268.

9.Zhao S , Chai H , Liu Y ,et al. Earthworms significantly enhance the temperature sensitivity of soil organic matter decomposition: Insights into future soil carbon budgeting[J].Agricultural and Forest Meteorology, 2025, 362.DOI:10.1016/j.agrformet.2025.110384.

10.Liu M, Yu Y, Liu Y, et al. Effects of polyethylene and poly (butyleneadipate-co-terephthalate) contamination on soil respiration and carbon sequestration[J]. Environmental Pollution, 2025, 364: 125315. 

11.Zhou, X. ,  Feng, Z. ,  Yao, Y. ,  Liu, R. ,  Shao, J. , &  Jia, S. , et al. Nitrogen input alleviates the priming effects of biochar addition on soil organic carbon decomposition. [J].  Soil Biology and Biochemistry, 2025, 202.

12.You M, Guo D, Shi H, et al. Microbial nutrient limitations and chemical composition of soil organic carbon regulate the organic carbon mineralization and temperature sensitivity in forest and grassland soils[J]. Plant and Soil, 2025: 1-18.

13.Wang C, Ren J, Cui Y, et al. Grazing-N addition interactions drive soil carbon priming and balance via bacterial assimilation in a meadow steppe [J]. Journal of Applied Ecology, 2025.

2024年

14.Liu Y, Kumar A, Tiemann L K, et al. Substrate availability reconciles the contrasting temperature response of SOC mineralization in different soil profiles[J]. Journal of Soils & Sediments: Protection, Risk Assessment, & Remediation, 2024, 24(1).DOI:10.1007/s11368-023-03602-y.

15.Yuna Ning, Zhanyi Wang, Cuiping Gao, et al. Effects of Different Grazing Intensities on Soil Respiration Rate and Its Temperature Sensitivity in Desert Steppe. [J]. Acta Agrestia Sinica, 2024, 32(10):3233-3240.DOI:10.11733/j.issn.1007-0435.2024.10.024.

16.Liu R , Zhou X , He Y ,et al. A transition from arbuscular to ectomycorrhizal forests halts soil carbon sequestration during subtropical forest rewilding[J].Science of the Total Environment, 2024, 946.DOI:10.1016/j.scitotenv.2024.174330.

17.Kang Y, Shen L, Li C, et al. Effects of vegetation degradation on soil microbial communities and ecosystem multifunctionality in a karst region, southwest China[J]. Journal of Environmental Management, 2024, 363: 121395.

18.Jun Pan, Yuan Liu, Nianpeng He, Chao Li, Mingxu Li, Li Xu, Osbert Jianxin Sun. 2024. The influence of forest-to-cropland conversion on temperature sensitivity of soil microbial respiration across tropical to temperate zones. Soil Biology and Biochemistry, doi:10.1016/j. soilbio.2024.109322.

19.Zheng J, Mao X, van Groenigen K J, et al. Decoupling of soil carbon mineralization and microbial community composition across a climate gradient on the Tibetan Plateau[J]. Geoderma, 2024, 441: 116736.

2023年

20.Yuanhao Liu, Decheng Xiong, Chen Wu, Yun Wang, Debao Lin, Jinxue Huang. Effects of exogenous carbon input on soil carbon emissions in evergreen broad-leaved forests [J]. Journal of Forest & Environment，Vol 43(5)，DOI: 10.13324/j.cnki.jfcf.2023.05.006

21.Li C, Xiao C, Li M, et al. The quality and quantity of SOM determines the mineralization of recently added labile C and priming of native SOM in grazed grasslands[J]. Geoderma, 2023, 432: 116385.

22.Xiaoliang Ma, Shengjing Jiang, Zhiqi Zhang, Hao Wang, Chao Song, Jin-Sheng He. Long‐term collar deployment leads to bias in soil respiration measurements[J]. Methods in Ecology and Evolution, 2023, 14(3): 981-990.

23.Yanghui He, Xuhui Zhou, Zhen Jia, Lingyan Zhou, Hongyang Chen, Ruiqiang Liu, Zhenggang Du, Guiyao Zhou, Junjiong Shao, Junxia Ding, Kelong Chen, Iain P. Hartley. Apparent thermal acclimation of soil heterotrophic respiration mainly mediated by substrate availability[J]. Global Change Biology, 2023, 29(4): 1178-1187.

2022年

24.Mao X, Zheng J, Yu W, et al. Climate-induced shifts in composition and protection regulate temperature sensitivity of carbon decomposition through soil profile[J]. Soil Biology and Biochemistry, 2022, 172: 108743.

25.Pan J, He N, Liu Y, et al. Growing season average temperature range is the optimal choice for Q10 incubation experiments of SOM decomposition[J]. Ecological Indicators, 2022, 145: 109749.

26.Li C, Xiao C, Guenet B, et al. Short-term effects of labile organic C addition on soil microbial response to temperature in a temperate steppe[J]. Soil Biology and Biochemistry, 2022, 167: 108589.

2021年及以前

27.Jiang ZX, Bian HF, Xu L, He NP. 2021. Pulse effect of precipitation: spatial patterns and mechanisms of soil carbon emissions. Frontiers in Ecology and Evolution, 9: 673310.

28.Liu Y, Xu L, Zheng S, Chen Z, Cao YQ, Wen XF, He NP. 2021. Temperature sensitivity of soil microbial respiration in soils with lower substrate availability is enhanced more by labile carbon input. Soil Biology and Biochemistry, 154: 108148.

29.Bian HF, Zheng S, Liu Y, Xu L, Chen Z, He NP. 2020. Changes in soil organic matter decomposition rate and its temperature sensitivity along water table gradients in cold-temperate forest swamps. Catena, 194: 104684.

30.Xu M, Wu SS, Jiang ZX, Xu L, Li MX, Bian HF, He NP. 2020. Effect of pulse precipitation on soil CO2 release in different grassland types on the Tibetan Plateau. European Journal of Soil Biology, 101: 103250.

31.Liu Y, He NP, Xu L, Tian J, Gao Y, Zheng S, Wang Q, Wen XF, Xu XL, Yakov K. 2019. A new incubation and measurement approach to estimate the temperature response of soil organic matter decomposition. Soil Biology & Biochemistry, 138, 107596.

32.Yingqiu C, Zhen Z, Li X, et al. Temperature Affects new Carbon Input Utilization By Soil Microbes: Evidence Based on a Rapid δ13C Measurement Technology[J]. Journal of Resources and Ecology, 2019, 10(2): 202-212.

33.Cao Y, Xu L, Zhang Z, et al. Soil microbial metabolic quotient in inner mongolian grasslands: Patterns and influence factors[J]. Chinese Geographical Science, 2019, 29: 1001-1010.

34.Liu Y, He NP, Wen XF, Xu L, Sun XM, Yu GR, Liang LY, Schipper LA. 2018. The optimum temperature of soil microbial respiration: Patterns and controls. Soil Biology and Biochemistry, 121: 35-42.

35.Liu Y, Wen XF, Zhang YH, Tian J, Gao Y, Ostle NJ, Niu SL, Chen SP, Sun XM, He NP. 2018.Widespread asymmetric response of soil heterotrophic respiration to warming and cooling. Science of Total Environment, 635: 423-431.

36.Wang Q, He NP, Xu L, Zhou XH. 2018. Important interaction of chemicals, microbial biomass and dissolved substrates in the diel hysteresis loop of soil heterotrophic respiration. Plant and Soil, 428: 279-290.

37.Wang Q, He NP, Xu L, Zhou XH. 2018. Microbial properties regulate spatial variation in the differences in heterotrophic respiration and its temperature sensitivity between primary and secondary forests from tropical to cold-temperate zones. Agriculture and Forest Meteorology, 262, 81-88.

38.He N P, Liu Y, Xu L, Wen X F, Yu G R, Sun X M. Temperature sensitivity of soil organic matter decomposition:New insights into models of incubation and measurement. Acta Ecologica Sinica, 2018, 38(11): 4045-4051.

39.Li J, He NP, Xu L, Chai H, Liu Y, Wang DL, Wang L, Wei XH, Xue JY, Wen XF, Sun XM. 2017. Asymmetric responses of soil heterotrophic respiration to rising and decreasing temperatures. Soil Biology & Biochemistry, 106: 18-27.

40.Liu Y, He NP, Xu L, Niu SL, Yu GR, Sun XM, Wen XF. 2017. Regional variation in the temperature sensitivity of soil organic matter decomposition in China’s forests and grasslands. Global Change Biology, 23: 3393-3402.

41.Wang Q, He NP*, Liu Y, Li ML, Xu L. 2016. Strong pulse effects of precipitation event on soil microbial respiration in temperate forests. Geoderma, 275: 67-73.

42.Wang Q, He NP, Yu GR, Gao Y, Wen XF, Wang RF, Koerner SE, Yu Q*. 2016. Soil microbial respiration rate and temperature sensitivity along a north-south forest transect in eastern China: Patterns and influencing factors. Journal of Geophysical Research: Biogeosciences, 121: 399-410.

43.He NP, Wang RM, Dai JZ, Gao Y, Wen XF, Yu GR. 2013. Changes in the temperature sensitivity of SOM decomposition with grassland succession: Implications for soil C sequestration. Ecology and Evolution, 3: 5045-5054.